Plankton Safari

Fourier Optics

2d_fourier_beam_3.jpg


1 = Near-infrared LASER beam
2 = Focussing lens (inverted microscope objective)
3 = Pinhole (in our case 10 micron)
4 = Collimating lens
5 = Collimated (expanded) laser beam
6 = Volume of water with algae and animals
7 = Objective lens
8 = Plane of 2D Fourier transform with binary filter
9 = Camera


With an optical set-up like this we can see phase objects swimming in the expanded laser beam. Here is a good example. The seahorse catches one brine shrimp larva and we can see the suspended algae all at the same time.

eugene_hecht_4th.jpg


Eugene Hecht, OPTICS, 4th edition, would be the text book

For zooplankton specifically:
[1] Strickler, J.R. 1977. Observation of swimming performances of planktonic copepods. Limnol. Oceanogr. 22: 165-170.
[2] Strickler, J.R. and J.- S. Hwang. 1999. Matched Spatial Filters in Long Working Distance Microscopy of Phase Objects. In: Cheng, P.C., Hwang, P.P., Wu, J.L., Wang, G. and Kim H. [Eds.] Focus on Multidimensional Microscopy. World Scientific Publishing Pte. Ltd., River Edge, NJ. pp. 217-239.